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Millipore recombinant reelin amino acids 164–496
List of primary antibodies.

Recombinant Reelin Amino Acids 164–496, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/recombinant reelin amino acids 164–496/product/Millipore
Average 90 stars, based on 1 article reviews

recombinant reelin amino acids 164–496 - by Bioz Stars, 2026-04

90/100 stars

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1) Product Images from "Reelin Mediates Hippocampal Cajal-Retzius Cell Positioning and Infrapyramidal Blade Morphogenesis"

Article Title: Reelin Mediates Hippocampal Cajal-Retzius Cell Positioning and Infrapyramidal Blade Morphogenesis

Journal: Journal of Developmental Biology

doi: 10.3390/jdb8030020

Figure Legend Snippet: List of primary antibodies.

Techniques Used: Concentration Assay, Recombinant, Derivative Assay, Plasmid Preparation, Isolation

Lack of the Cajal-Retzius cells in the infrapyramidal blade. ( A ) The infrapyramidal blade (IPB) of dentate gyrus does not develop in Reln CTRdel , and this coincides with the absence of the RELN-positive cells (gray or red) in the prospective infrapyramidal blade. P7 brains were immunostained with anti-RELN antibodies ( n = 2 each). ( B ) Distribution of p73-expressing cells (gray or green) in the dentate gyrus at P7 ( n = 2 each). The number of p73-expressing cells is increased in the suprapyramidal blade (SPB). Nuclei were stained using Hoechst (blue). Scale bars, 250 µm. Arrows indicate the edge of Cajal-Retzius cell-containing regions, which are near the fimbrodentate junction (in the wild type) or the tip of truncated IPB (in the mutants).

Figure Legend Snippet: Lack of the Cajal-Retzius cells in the infrapyramidal blade. ( A ) The infrapyramidal blade (IPB) of dentate gyrus does not develop in Reln CTRdel , and this coincides with the absence of the RELN-positive cells (gray or red) in the prospective infrapyramidal blade. P7 brains were immunostained with anti-RELN antibodies ( n = 2 each). ( B ) Distribution of p73-expressing cells (gray or green) in the dentate gyrus at P7 ( n = 2 each). The number of p73-expressing cells is increased in the suprapyramidal blade (SPB). Nuclei were stained using Hoechst (blue). Scale bars, 250 µm. Arrows indicate the edge of Cajal-Retzius cell-containing regions, which are near the fimbrodentate junction (in the wild type) or the tip of truncated IPB (in the mutants).

Techniques Used: Expressing, Staining

Absence of the neurogenic cluster at the fimbriodentate junction. ( A ) Distribution of the BrdU (red) or Ki67-positive cells (green) in the dentate gyrus at P4, after 24 h from BrdU injection. A cluster of dividing cells is present at the fimbriodentate junction of the wild-type (white circle, a). A Reln CTRdel mutant does not have this cluster (a’). Compared with a corresponding region of the wild type (b), abnormal accumulation of the dividing cells are apparent in the subpial surface of Reln CTRdel (b’). Enlarged images of marked regions (a, a’, b, b’) are shown below. In the mutant, Ki67-positive cells are closely located on top of BrdU-positive cells (a’ and b’). Scale bars, 100 µm. Scale bars in the enlarged images, 25 µm. Nuclei were stained using Hoechst (blue). ( B ) The number of total BrdU-positive cells is not significantly different ( p = 0.8182; boxplot, median ± IQR; whiskers, min and max). ( C ) The percentage of BrdU/Ki67 double-positive cells was reduced ( p = 0.0022; boxplot, median ± IQR; whiskers, min and max). This represents a population of cells that reentered the cell cycle.

Figure Legend Snippet: Absence of the neurogenic cluster at the fimbriodentate junction. ( A ) Distribution of the BrdU (red) or Ki67-positive cells (green) in the dentate gyrus at P4, after 24 h from BrdU injection. A cluster of dividing cells is present at the fimbriodentate junction of the wild-type (white circle, a). A Reln CTRdel mutant does not have this cluster (a’). Compared with a corresponding region of the wild type (b), abnormal accumulation of the dividing cells are apparent in the subpial surface of Reln CTRdel (b’). Enlarged images of marked regions (a, a’, b, b’) are shown below. In the mutant, Ki67-positive cells are closely located on top of BrdU-positive cells (a’ and b’). Scale bars, 100 µm. Scale bars in the enlarged images, 25 µm. Nuclei were stained using Hoechst (blue). ( B ) The number of total BrdU-positive cells is not significantly different ( p = 0.8182; boxplot, median ± IQR; whiskers, min and max). ( C ) The percentage of BrdU/Ki67 double-positive cells was reduced ( p = 0.0022; boxplot, median ± IQR; whiskers, min and max). This represents a population of cells that reentered the cell cycle.

Techniques Used: Injection, Mutagenesis, Staining

Cell death analysis. Activated caspase-3 (green) staining at P4 showed no abnormal increase in cell death in Reln CTRdel hippocampus. Nuclei were stained using Hoechst (blue).

Figure Legend Snippet: Cell death analysis. Activated caspase-3 (green) staining at P4 showed no abnormal increase in cell death in Reln CTRdel hippocampus. Nuclei were stained using Hoechst (blue).

Techniques Used: Staining

Lack of intermediate progenitor accumulation at the fimbriodentate junction. In wild-type mice, Tbr2-positive cells (gray or green) accumulate at the fimbriodentate junction (arrowheads) starting at P1 ( n = 2 each) and more clearly at P4 ( n = 2 each) and P7 ( n = 2 each). This accumulation at FDJ is less apparent in the mutant, and the labeled cells are spread out along the ventricular surface. Enlarged imaged of the hilus at P7 are shown in the lower panel. Reln CTRdel mice have fewer Tbr2-labeled cells in the hilus (H) compared to wild-type mice; it appears as if many cells are still confined to the ventricular surface. Nuclei were stained using Hoechst (blue). Scale bars, 100 µm.

Figure Legend Snippet: Lack of intermediate progenitor accumulation at the fimbriodentate junction. In wild-type mice, Tbr2-positive cells (gray or green) accumulate at the fimbriodentate junction (arrowheads) starting at P1 ( n = 2 each) and more clearly at P4 ( n = 2 each) and P7 ( n = 2 each). This accumulation at FDJ is less apparent in the mutant, and the labeled cells are spread out along the ventricular surface. Enlarged imaged of the hilus at P7 are shown in the lower panel. Reln CTRdel mice have fewer Tbr2-labeled cells in the hilus (H) compared to wild-type mice; it appears as if many cells are still confined to the ventricular surface. Nuclei were stained using Hoechst (blue). Scale bars, 100 µm.

Techniques Used: Mutagenesis, Labeling, Staining

Abnormal distribution of the granule neurons. Granule neuron precursor markers (gray or green), NeuroD ( A ) and Prox1 ( B ), staining indicate the presence of abnormal progenitor population in the hilus (H) of Reln CTRdel at P7 ( n = 2 each). Nuclei were stained using Hoechst (blue). Enlarged images are shown in lower panels. Scale bars, 100 µm.

Figure Legend Snippet: Abnormal distribution of the granule neurons. Granule neuron precursor markers (gray or green), NeuroD ( A ) and Prox1 ( B ), staining indicate the presence of abnormal progenitor population in the hilus (H) of Reln CTRdel at P7 ( n = 2 each). Nuclei were stained using Hoechst (blue). Enlarged images are shown in lower panels. Scale bars, 100 µm.

Techniques Used: Staining

Lack of the secondary glial scaffold formation in the IPB. ( A ) At P1 when the secondary glial scaffold formation begins in the IPB, the mutant mice lack dense GFAP-stained fibers (gray or green, marked region) that are apparent near the fimbriodentate junction of wild-type mice ( n = 2 each). Note the premature secondary glial scaffold can be already seen in the SPB of both wild-type and mutant. ( B ) At P1, BLBP-positive cell bodies (gray or red) appear near the IPB in wild-type mice ( n = 2 each). Fewer BLBP-positive cells are found near the IPB of Reln CTRdel mice. ( C ) Images of GFAP-stained (gray or green) dentate gyrus at P7 (left, n = 2 each). Enlarged images of the boxed fimbriodentate junction (FDJ) regions are shown in the center. In wild-type mice, GFAP-positive fibers are surrounding the growing tip of the infrapyramidal blade at the fimbriodentate junction (arrow). This structure is absent in the mutant. The secondary glia scaffold in the SPB is relatively normal (right, higher-magnification images). ( D ) At P4, a stream of migrating cells with elongated morphology is absent in the mutant fimbriodentate junction ( n = 1 each). Enlarged images of the fimbriodentate junction regions are shown on the right. Nissl stain. ( E ) At P7, abnormal distribution of BLBP-positive cells (gray or red) in the hilus become more evident ( n = 2 each). The cells are directed toward the SPB in the mutant. Nuclei were stained using Hoechst (blue). Scale bars, 100 µm. Enlarged images of ( A ) (color panel), ( B ) (color panel), ( D ) (right panel), and ( E ) (color panel) can be found in .

Figure Legend Snippet: Lack of the secondary glial scaffold formation in the IPB. ( A ) At P1 when the secondary glial scaffold formation begins in the IPB, the mutant mice lack dense GFAP-stained fibers (gray or green, marked region) that are apparent near the fimbriodentate junction of wild-type mice ( n = 2 each). Note the premature secondary glial scaffold can be already seen in the SPB of both wild-type and mutant. ( B ) At P1, BLBP-positive cell bodies (gray or red) appear near the IPB in wild-type mice ( n = 2 each). Fewer BLBP-positive cells are found near the IPB of Reln CTRdel mice. ( C ) Images of GFAP-stained (gray or green) dentate gyrus at P7 (left, n = 2 each). Enlarged images of the boxed fimbriodentate junction (FDJ) regions are shown in the center. In wild-type mice, GFAP-positive fibers are surrounding the growing tip of the infrapyramidal blade at the fimbriodentate junction (arrow). This structure is absent in the mutant. The secondary glia scaffold in the SPB is relatively normal (right, higher-magnification images). ( D ) At P4, a stream of migrating cells with elongated morphology is absent in the mutant fimbriodentate junction ( n = 1 each). Enlarged images of the fimbriodentate junction regions are shown on the right. Nissl stain. ( E ) At P7, abnormal distribution of BLBP-positive cells (gray or red) in the hilus become more evident ( n = 2 each). The cells are directed toward the SPB in the mutant. Nuclei were stained using Hoechst (blue). Scale bars, 100 µm. Enlarged images of ( A ) (color panel), ( B ) (color panel), ( D ) (right panel), and ( E ) (color panel) can be found in .

Techniques Used: Mutagenesis, Staining

A model of the infrapyramidal malformation. During the first postnatal week, Cajal-Retzius cells disperse along the subpial surface of the IPB. The progenitors migrate along the dentate migratory stream, then along the densely organized glia scaffold at the fimbriodentate junction, proliferate at the tip and extend the IPB. In Reln CTRdel mutant, Cajal-Retzius cells overmigrate to the SPB and are absent in the subpial surface of the IPB. Without proper RELN signal, the glia scaffolds cannot become organized at the fimbriodentate junction, the progenitor cells fail to migrate toward the hilus, and proliferate within the subpial surface. The mutant dentate gyrus fails to form the outer shell in the IPB, which affects subsequent neurogenesis events in the dentate gyrus.

Figure Legend Snippet: A model of the infrapyramidal malformation. During the first postnatal week, Cajal-Retzius cells disperse along the subpial surface of the IPB. The progenitors migrate along the dentate migratory stream, then along the densely organized glia scaffold at the fimbriodentate junction, proliferate at the tip and extend the IPB. In Reln CTRdel mutant, Cajal-Retzius cells overmigrate to the SPB and are absent in the subpial surface of the IPB. Without proper RELN signal, the glia scaffolds cannot become organized at the fimbriodentate junction, the progenitor cells fail to migrate toward the hilus, and proliferate within the subpial surface. The mutant dentate gyrus fails to form the outer shell in the IPB, which affects subsequent neurogenesis events in the dentate gyrus.

Techniques Used: Mutagenesis

A comparison of the infrapyramidal blade (IPB) malformation between Reln CTRdel and Apoer2 null mutants. Apoer2 null mutant dentate gyrus also has a truncated IPB although not as severe as Reln CTRdel . This phenotype appears more pronounced in the posterior sections; images of the entire hippocampal region are displayed to show that cross-sections of the similar region were compared. Scale bars, 500 µm.

Figure Legend Snippet: A comparison of the infrapyramidal blade (IPB) malformation between Reln CTRdel and Apoer2 null mutants. Apoer2 null mutant dentate gyrus also has a truncated IPB although not as severe as Reln CTRdel . This phenotype appears more pronounced in the posterior sections; images of the entire hippocampal region are displayed to show that cross-sections of the similar region were compared. Scale bars, 500 µm.

Techniques Used: Comparison, Mutagenesis

Image Search Results

Journal: Journal of Developmental Biology

Article Title: Reelin Mediates Hippocampal Cajal-Retzius Cell Positioning and Infrapyramidal Blade Morphogenesis

doi: 10.3390/jdb8030020

Figure Lengend Snippet: List of primary antibodies.

Article Snippet: Reelin , Recombinant reelin amino acids 164–496 , Millipore, mouse, monoclonal, Cat# MAB5364, clone G10, RRID:AB_2179313 , 1 mg/mL, 1:1000.

Techniques: Concentration Assay, Recombinant, Derivative Assay, Plasmid Preparation, Isolation

Journal: Journal of Developmental Biology

Article Title: Reelin Mediates Hippocampal Cajal-Retzius Cell Positioning and Infrapyramidal Blade Morphogenesis

doi: 10.3390/jdb8030020

Figure Lengend Snippet: Lack of the Cajal-Retzius cells in the infrapyramidal blade. ( A ) The infrapyramidal blade (IPB) of dentate gyrus does not develop in Reln CTRdel , and this coincides with the absence of the RELN-positive cells (gray or red) in the prospective infrapyramidal blade. P7 brains were immunostained with anti-RELN antibodies ( n = 2 each). ( B ) Distribution of p73-expressing cells (gray or green) in the dentate gyrus at P7 ( n = 2 each). The number of p73-expressing cells is increased in the suprapyramidal blade (SPB). Nuclei were stained using Hoechst (blue). Scale bars, 250 µm. Arrows indicate the edge of Cajal-Retzius cell-containing regions, which are near the fimbrodentate junction (in the wild type) or the tip of truncated IPB (in the mutants).

Article Snippet: Reelin , Recombinant reelin amino acids 164–496 , Millipore, mouse, monoclonal, Cat# MAB5364, clone G10, RRID:AB_2179313 , 1 mg/mL, 1:1000.

Techniques: Expressing, Staining

Journal: Journal of Developmental Biology

Article Title: Reelin Mediates Hippocampal Cajal-Retzius Cell Positioning and Infrapyramidal Blade Morphogenesis

doi: 10.3390/jdb8030020

Figure Lengend Snippet: Absence of the neurogenic cluster at the fimbriodentate junction. ( A ) Distribution of the BrdU (red) or Ki67-positive cells (green) in the dentate gyrus at P4, after 24 h from BrdU injection. A cluster of dividing cells is present at the fimbriodentate junction of the wild-type (white circle, a). A Reln CTRdel mutant does not have this cluster (a’). Compared with a corresponding region of the wild type (b), abnormal accumulation of the dividing cells are apparent in the subpial surface of Reln CTRdel (b’). Enlarged images of marked regions (a, a’, b, b’) are shown below. In the mutant, Ki67-positive cells are closely located on top of BrdU-positive cells (a’ and b’). Scale bars, 100 µm. Scale bars in the enlarged images, 25 µm. Nuclei were stained using Hoechst (blue). ( B ) The number of total BrdU-positive cells is not significantly different ( p = 0.8182; boxplot, median ± IQR; whiskers, min and max). ( C ) The percentage of BrdU/Ki67 double-positive cells was reduced ( p = 0.0022; boxplot, median ± IQR; whiskers, min and max). This represents a population of cells that reentered the cell cycle.

Article Snippet: Reelin , Recombinant reelin amino acids 164–496 , Millipore, mouse, monoclonal, Cat# MAB5364, clone G10, RRID:AB_2179313 , 1 mg/mL, 1:1000.

Techniques: Injection, Mutagenesis, Staining

Journal: Journal of Developmental Biology

Article Title: Reelin Mediates Hippocampal Cajal-Retzius Cell Positioning and Infrapyramidal Blade Morphogenesis

doi: 10.3390/jdb8030020

Figure Lengend Snippet: Cell death analysis. Activated caspase-3 (green) staining at P4 showed no abnormal increase in cell death in Reln CTRdel hippocampus. Nuclei were stained using Hoechst (blue).

Article Snippet: Reelin , Recombinant reelin amino acids 164–496 , Millipore, mouse, monoclonal, Cat# MAB5364, clone G10, RRID:AB_2179313 , 1 mg/mL, 1:1000.

Techniques: Staining

Journal: Journal of Developmental Biology

Article Title: Reelin Mediates Hippocampal Cajal-Retzius Cell Positioning and Infrapyramidal Blade Morphogenesis

doi: 10.3390/jdb8030020

Figure Lengend Snippet: Lack of intermediate progenitor accumulation at the fimbriodentate junction. In wild-type mice, Tbr2-positive cells (gray or green) accumulate at the fimbriodentate junction (arrowheads) starting at P1 ( n = 2 each) and more clearly at P4 ( n = 2 each) and P7 ( n = 2 each). This accumulation at FDJ is less apparent in the mutant, and the labeled cells are spread out along the ventricular surface. Enlarged imaged of the hilus at P7 are shown in the lower panel. Reln CTRdel mice have fewer Tbr2-labeled cells in the hilus (H) compared to wild-type mice; it appears as if many cells are still confined to the ventricular surface. Nuclei were stained using Hoechst (blue). Scale bars, 100 µm.

Article Snippet: Reelin , Recombinant reelin amino acids 164–496 , Millipore, mouse, monoclonal, Cat# MAB5364, clone G10, RRID:AB_2179313 , 1 mg/mL, 1:1000.

Techniques: Mutagenesis, Labeling, Staining

Journal: Journal of Developmental Biology

Article Title: Reelin Mediates Hippocampal Cajal-Retzius Cell Positioning and Infrapyramidal Blade Morphogenesis

doi: 10.3390/jdb8030020

Figure Lengend Snippet: Abnormal distribution of the granule neurons. Granule neuron precursor markers (gray or green), NeuroD ( A ) and Prox1 ( B ), staining indicate the presence of abnormal progenitor population in the hilus (H) of Reln CTRdel at P7 ( n = 2 each). Nuclei were stained using Hoechst (blue). Enlarged images are shown in lower panels. Scale bars, 100 µm.

Article Snippet: Reelin , Recombinant reelin amino acids 164–496 , Millipore, mouse, monoclonal, Cat# MAB5364, clone G10, RRID:AB_2179313 , 1 mg/mL, 1:1000.

Techniques: Staining

Journal: Journal of Developmental Biology

Article Title: Reelin Mediates Hippocampal Cajal-Retzius Cell Positioning and Infrapyramidal Blade Morphogenesis

doi: 10.3390/jdb8030020

Figure Lengend Snippet: Lack of the secondary glial scaffold formation in the IPB. ( A ) At P1 when the secondary glial scaffold formation begins in the IPB, the mutant mice lack dense GFAP-stained fibers (gray or green, marked region) that are apparent near the fimbriodentate junction of wild-type mice ( n = 2 each). Note the premature secondary glial scaffold can be already seen in the SPB of both wild-type and mutant. ( B ) At P1, BLBP-positive cell bodies (gray or red) appear near the IPB in wild-type mice ( n = 2 each). Fewer BLBP-positive cells are found near the IPB of Reln CTRdel mice. ( C ) Images of GFAP-stained (gray or green) dentate gyrus at P7 (left, n = 2 each). Enlarged images of the boxed fimbriodentate junction (FDJ) regions are shown in the center. In wild-type mice, GFAP-positive fibers are surrounding the growing tip of the infrapyramidal blade at the fimbriodentate junction (arrow). This structure is absent in the mutant. The secondary glia scaffold in the SPB is relatively normal (right, higher-magnification images). ( D ) At P4, a stream of migrating cells with elongated morphology is absent in the mutant fimbriodentate junction ( n = 1 each). Enlarged images of the fimbriodentate junction regions are shown on the right. Nissl stain. ( E ) At P7, abnormal distribution of BLBP-positive cells (gray or red) in the hilus become more evident ( n = 2 each). The cells are directed toward the SPB in the mutant. Nuclei were stained using Hoechst (blue). Scale bars, 100 µm. Enlarged images of ( A ) (color panel), ( B ) (color panel), ( D ) (right panel), and ( E ) (color panel) can be found in .

Article Snippet: Reelin , Recombinant reelin amino acids 164–496 , Millipore, mouse, monoclonal, Cat# MAB5364, clone G10, RRID:AB_2179313 , 1 mg/mL, 1:1000.

Techniques: Mutagenesis, Staining

Journal: Journal of Developmental Biology

Article Title: Reelin Mediates Hippocampal Cajal-Retzius Cell Positioning and Infrapyramidal Blade Morphogenesis

doi: 10.3390/jdb8030020

Figure Lengend Snippet: A model of the infrapyramidal malformation. During the first postnatal week, Cajal-Retzius cells disperse along the subpial surface of the IPB. The progenitors migrate along the dentate migratory stream, then along the densely organized glia scaffold at the fimbriodentate junction, proliferate at the tip and extend the IPB. In Reln CTRdel mutant, Cajal-Retzius cells overmigrate to the SPB and are absent in the subpial surface of the IPB. Without proper RELN signal, the glia scaffolds cannot become organized at the fimbriodentate junction, the progenitor cells fail to migrate toward the hilus, and proliferate within the subpial surface. The mutant dentate gyrus fails to form the outer shell in the IPB, which affects subsequent neurogenesis events in the dentate gyrus.

Article Snippet: Reelin , Recombinant reelin amino acids 164–496 , Millipore, mouse, monoclonal, Cat# MAB5364, clone G10, RRID:AB_2179313 , 1 mg/mL, 1:1000.

Techniques: Mutagenesis

Journal: Journal of Developmental Biology

Article Title: Reelin Mediates Hippocampal Cajal-Retzius Cell Positioning and Infrapyramidal Blade Morphogenesis

doi: 10.3390/jdb8030020

Figure Lengend Snippet: A comparison of the infrapyramidal blade (IPB) malformation between Reln CTRdel and Apoer2 null mutants. Apoer2 null mutant dentate gyrus also has a truncated IPB although not as severe as Reln CTRdel . This phenotype appears more pronounced in the posterior sections; images of the entire hippocampal region are displayed to show that cross-sections of the similar region were compared. Scale bars, 500 µm.

Article Snippet: Reelin , Recombinant reelin amino acids 164–496 , Millipore, mouse, monoclonal, Cat# MAB5364, clone G10, RRID:AB_2179313 , 1 mg/mL, 1:1000.

Techniques: Comparison, Mutagenesis

Journal: Frontiers in Neuroanatomy

Article Title: Distribution of interneurons in the CA2 region of the rat hippocampus

doi: 10.3389/fnana.2014.00104

Figure Lengend Snippet: Primary antibody list .

Article Snippet: Reelin , Recombinant reelin amino acids 164-496 , Millipore , Mouse , MAB5364 , 1:10000.

Techniques: Purification, Recombinant

Molecular characterization of interneurons in the CA2 region of the hippocampus . Percentage of inhibitory cells that express parvalbumin (PV-black), cholecystokinin (CCK-gray), calbindin (CB-turquoise), Vasoactive Intestinal Peptide (VIP-light blue), somatostatin (SOM-purple), Reelin (dark blue), Neuropeptide Y (NPY-green), and calretinin (CR-pink) in each of the CA regions, CA1, CA2, and CA3. Total numbers of inhibitory neurons were obtained from the same sections labeled with anti-GAD67 antibodies.

Journal: Frontiers in Neuroanatomy

Article Title: Distribution of interneurons in the CA2 region of the rat hippocampus

doi: 10.3389/fnana.2014.00104

Figure Lengend Snippet: Molecular characterization of interneurons in the CA2 region of the hippocampus . Percentage of inhibitory cells that express parvalbumin (PV-black), cholecystokinin (CCK-gray), calbindin (CB-turquoise), Vasoactive Intestinal Peptide (VIP-light blue), somatostatin (SOM-purple), Reelin (dark blue), Neuropeptide Y (NPY-green), and calretinin (CR-pink) in each of the CA regions, CA1, CA2, and CA3. Total numbers of inhibitory neurons were obtained from the same sections labeled with anti-GAD67 antibodies.

Article Snippet: Reelin , Recombinant reelin amino acids 164-496 , Millipore , Mouse , MAB5364 , 1:10000.

Techniques: Labeling

Densities of immunolabeled interneurons in all layers of the CA1, CA2, and CA3 regions . Cell densities of inhibitory cells (number of cell bodies per volume) that express parvalbumin (PV-black), calbindin (CB-turquoise), calretinin (CR-pink), Reelin (dark blue), cholecystokinin (CCK-gray), Vasoactive Intestinal Peptide (VIP-light blue), Neuropeptide Y (NPY-green), and somatostatin (SOM-purple) in stratum oriens (SO), stratum pyramidale (SP), Stratum radiatum (SR), and stratum lacunosum moleculare (SLM) of CA1, CA2, and CA3. There was a higher density of PV- and Reelin-positive neurons in SO in CA2 than in CA1 and CA3 (MWU P < 0.05). SP of the CA2 region contained a higher density of PV-, Reelin- and CB-positive interneurons than in CA1 and CA3 (MWU P < 0.05). There were also a higher number of VIP-positive somata in SP in CA2 than in CA3 and a larger density of SOM-positive cells in SP in CA2 than in CA1 (MWU P < 0.05). A higher density of Reelin- and SOM-positive neurons was found in SR in CA2 than in CA1 (MWU P < 0.05). * P = 0.01 to 0.05; ** P = 0.001 to 0.01; *** P < 0.001.

Journal: Frontiers in Neuroanatomy

Article Title: Distribution of interneurons in the CA2 region of the rat hippocampus

doi: 10.3389/fnana.2014.00104

Figure Lengend Snippet: Densities of immunolabeled interneurons in all layers of the CA1, CA2, and CA3 regions . Cell densities of inhibitory cells (number of cell bodies per volume) that express parvalbumin (PV-black), calbindin (CB-turquoise), calretinin (CR-pink), Reelin (dark blue), cholecystokinin (CCK-gray), Vasoactive Intestinal Peptide (VIP-light blue), Neuropeptide Y (NPY-green), and somatostatin (SOM-purple) in stratum oriens (SO), stratum pyramidale (SP), Stratum radiatum (SR), and stratum lacunosum moleculare (SLM) of CA1, CA2, and CA3. There was a higher density of PV- and Reelin-positive neurons in SO in CA2 than in CA1 and CA3 (MWU P < 0.05). SP of the CA2 region contained a higher density of PV-, Reelin- and CB-positive interneurons than in CA1 and CA3 (MWU P < 0.05). There were also a higher number of VIP-positive somata in SP in CA2 than in CA3 and a larger density of SOM-positive cells in SP in CA2 than in CA1 (MWU P < 0.05). A higher density of Reelin- and SOM-positive neurons was found in SR in CA2 than in CA1 (MWU P < 0.05). * P = 0.01 to 0.05; ** P = 0.001 to 0.01; *** P < 0.001.

Article Snippet: Reelin , Recombinant reelin amino acids 164-496 , Millipore , Mouse , MAB5364 , 1:10000.

Techniques: Immunolabeling

Distribution of Reelin-immunopositive interneurons in CA2. (A) Immunoperoxidase staining for Reelin. The borders of the CA2 region was defined by superimposing the reelin staining and the staining for PCP4 (right panel) and represented with dotted lines. Some reelin-immunopositive cell bodies are indicated with white arrows on the top left panel. Example of the reelin immunoperoxidase staining in SR of the CA2 region is shown in the bottom left panel. Scale bars 1 mm (right panel), 200 μm (top left panel), and 50 μm (bottom left panel). SO, Stratum Oriens; SP, stratum pyramidale; SR, stratum Radiatum; SLM, Stratum Lacunosum Moleculare; SL, Stratum Lucidum. (B) Distribution of reelin-immunopositive somata in CA1, CA2, and CA3 (superimposition of five 50 μm-thick sections). (C) Distribution of reelin-positive neurons in all layers of CA1, CA2, and CA3. Numbers are expressed as percentage of GAD-immunopositive neurons (5 sections- n = 7). Reelin-positive somata were distributed in all layers of the three CA regions. However, there was a larger proportion of GAD-positive cells that were reelin-positive in SP and SR of the CA2 region than in CA1 and CA3 (MWU P < 0.05). Reconstructions are not shown because this antibody does not label dendrites adequately. ** P = 0.001 and 0.01.

Journal: Frontiers in Neuroanatomy

Article Title: Distribution of interneurons in the CA2 region of the rat hippocampus

doi: 10.3389/fnana.2014.00104

Figure Lengend Snippet: Distribution of Reelin-immunopositive interneurons in CA2. (A) Immunoperoxidase staining for Reelin. The borders of the CA2 region was defined by superimposing the reelin staining and the staining for PCP4 (right panel) and represented with dotted lines. Some reelin-immunopositive cell bodies are indicated with white arrows on the top left panel. Example of the reelin immunoperoxidase staining in SR of the CA2 region is shown in the bottom left panel. Scale bars 1 mm (right panel), 200 μm (top left panel), and 50 μm (bottom left panel). SO, Stratum Oriens; SP, stratum pyramidale; SR, stratum Radiatum; SLM, Stratum Lacunosum Moleculare; SL, Stratum Lucidum. (B) Distribution of reelin-immunopositive somata in CA1, CA2, and CA3 (superimposition of five 50 μm-thick sections). (C) Distribution of reelin-positive neurons in all layers of CA1, CA2, and CA3. Numbers are expressed as percentage of GAD-immunopositive neurons (5 sections- n = 7). Reelin-positive somata were distributed in all layers of the three CA regions. However, there was a larger proportion of GAD-positive cells that were reelin-positive in SP and SR of the CA2 region than in CA1 and CA3 (MWU P < 0.05). Reconstructions are not shown because this antibody does not label dendrites adequately. ** P = 0.001 and 0.01.

Article Snippet: Reelin , Recombinant reelin amino acids 164-496 , Millipore , Mouse , MAB5364 , 1:10000.

Techniques: Immunoperoxidase Staining, Staining

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Structured Review

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1) Product Images from "Reelin Mediates Hippocampal Cajal-Retzius Cell Positioning and Infrapyramidal Blade Morphogenesis"

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